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PURIFICATION OF GONADOTROPHIN FROM HUMAN MENOPAUSAL URINE

P DONINI, R MONTEZEMOLO, D PUZZUOLI

  • Istituto di Farmacologia Traslazionale ROR

Acta Endocrinologica, 45(3), 321-328

DOI 10.1530/acta.0.0450321 PMID 14131763

Abstract

Gonadotrophins were prepared from human postmenopausal urine with the kaolin-acetone method and purified by adsorption of impurities on diethylamine ethyl cellulose (DEAE-C).

The last step, namely chromatography on the column of permutit, resulted in a significantly improved specific activity.

The immunoelectrophoresis showed that the purified HMG is not homogeneous and that the constituents are glycoproteins which migrate into the region α1, α2and β globulins.

Topics

Reproductive Endocrinology > Gonadotropins > Purification MethodsInfertility > Ovulation Induction > Gonadotropin PreparationDiagnostics > Laboratory Methods > Protein Purification
human menopausal gonadotropin purification methods, HMG extraction postmenopausal urine kaolin acetone, Donini gonadotrophin purification menopausal urine, DEAE cellulose chromatography gonadotropin purification, human menopausal gonadotropin glycoprotein characterization, FSH LH purification urine postmenopausal, immunoelectrophoresis gonadotropin glycoprotein analysis, permutit chromatography gonadotropin specific activity, gonadotropin preparation fertility treatment historical
PMID 14131763 14131763 DOI 10.1530/acta.0.0450321 10.1530/acta.0.0450321

Cite this article

Donini, P., Puzzuoli, D., & Montezemolo, R. (1964). Purification of gonadotrophin from human menopausal urine. *Acta endocrinologica*, *45*(3), 321-328. https://doi.org/10.1530/acta.0.0450321

Keywords

Alpha-Globulins, Beta-Globulins, Chromatography, Glycoproteins, Gonadotropins, Gonadotropins, Pituitary, Immunoelectrophoresis, Menopause, Serum Globulins, Urine, Alpha-Globulins, Beta-Globulins, Glycoproteins, Gonadotropins, Gonadotropins, Pituitary, Serum Globulins, ALPHA GLOBULIN, BETA GLOBULIN, CHROMATOGRAPHY, GLYCOPROTEINS, GONADOTROPINS, PITUITARY, IMMUNOELECTROPHORESIS, MENOPAUSE, SERUM GLOBULINS, URINE

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