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Purification of gonadotrophin from human menopausal urine

Donini P et al., 1964

Acta endocrinologica, 45(3), 321-328

DOI 10.1530/acta.0.0450321 PMID 14131763 Source

Abstract

Gonadotrophins were prepared from human postmenopausal urine with the kaolin-acetone method and purified by adsorption of impurities on diethylamine ethyl cellulose (DEAE-C). The last step, namely chromatography on the column of permutit, resulted in a significantly improved specific activity. The immunoelectrophoresis showed that the purified HMG is not homogeneous and that the constituents are glycoproteins which migrate into the region α1, α2and β globulins.

Topics

Reproductive Endocrinology > Ovulation > InductionGeneral OB/GYN > Clinical Practice > Standards
human menopausal gonadotropin purification, hmg extraction methods, postmenopausal urine gonadotropin isolation, gonadotropin biochemistry purification, hmg production techniques historical, menotropins purification process, fertility hormone extraction methods, gonadotropin glycoprotein characterization

Cite this article

Donini, P., Puzzuoli, D., & Montezemolo, R. (1964). Purification of gonadotrophin from human menopausal urine. *Acta endocrinologica*, *45*(3), 321-328. https://doi.org/10.1530/acta.0.0450321

Keywords

Alpha-Globulins, Beta-Globulins, Chromatography, Glycoproteins, Gonadotropins, Pituitary, Immunoelectrophoresis, Menopause, Serum Globulins, Urine, Alpha Globulin, Beta Globulin

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