Plasma progesterone levels in normal pregnancy, labor, and the puerperium. I. Method of assay

American journal of obstetrics and gynecology, 101(8), 1054-1057

DOI 10.1016/0002-9378(68)90347-5 PMID 5690916 Source

A method to measure plasma progesterone by gas-liquid chromatography is presented. The procedure allows for the preparation of a plasma progesterone extract clean enough for accurate submicrogram quantification by gas-liquid chromatography. In a gas chromatograph equipped with a hydrogen flame detector, the instrument modifications and operating conditions as outlined make possible the measurement of 0.001 μg (1 ng.) of progesterone standard on a stable recorder base line. In biologic samples the sensitivity of the assay is in the order of 0.01 μg (10 ng.) of progesterone.

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Yannone, M. E. (1968). Plasma progesterone levels in normal pregnancy, labor, and the puerperium. I. Method of assay. *American journal of obstetrics and gynecology*, *101*(8), 1054-1057. https://doi.org/10.1016/0002-9378(68)90347-5