0 Ask Beta Account

Isolation and characterization of 5alpha-pregnane-3,20-dione and progesterone in pepipheral blood of pregnant women. measurement throughout pregnancy

Gomez-Sanchez C, P C MacDonald

  • The University of Texas Southwestern Medical Center ROR

Gynecologic Investigation, 6(5), 291-306

Source

Abstract

5alpha-Pregnane-3,20-dione and progesterone were isolated from a pregnancy plasma pool and were identified by using a combination of chromatographic techniques and mass spectrometry. Antibodies to progesterone were obtained in rabbits by immunization with progesterone-1alpha-carboxyethyl-thioether-thyroglobulin. The raised antibodies were of high affinity and one of them cross-reacted (137%) with 5alpha-pregnane-3,20-dione. This property was used to develop radioimmunoassays for measuring circulating levels of both progesterone and 5alpha-pregnane-3,20-dione in pregnancy plasma. The levels of both progesterone and 5alpha-pregnane-3,20-dione increase throughout pregnancy, but a highly significant increase is observed only after the 32nd week of gestation.

Topics

Pregnancy > Hormonal Changes > Progesterone LevelsDiagnostics > Hormone Assays > Radioimmunoassay DevelopmentReproductive Endocrinology > Steroid Metabolism > Progesterone Metabolites
progesterone levels throughout pregnancy measurement, 5alpha-pregnane-3,20-dione pregnancy plasma isolation, progesterone radioimmunoassay pregnancy peripheral blood, steroid hormone increase third trimester 32 weeks gestation, Milewich MacDonald progesterone pregnancy plasma, 5alpha-dihydroprogesterone pregnancy characterization mass spectrometry, progesterone antibody cross-reactivity radioimmunoassay development, circulating progesterone concentration pregnancy progression, pregnane steroid metabolites peripheral blood pregnant women, progesterone immunization thyroglobulin antibody pregnancy assay

Cite this article

Milewich, L., Gomez-Sanchez, C., Madden, J. D., & MacDonald, P. C. (1975). Isolation and characterization of 5alpha-pregnane-3,20-dione and progesterone in pepipheral blood of pregnant women. measurement throughout pregnancy. *Gynecologic investigation*, *6*(5), 291-306.

Keywords

Animals, Antibodies, Female, Humans, Pregnancy, Pregnancy Trimester, Second, Pregnancy Trimester, Third, Pregnanediones/blood/isolation & Purification, Progesterone/blood/immunology/isolation & Purification, Rabbits/immunology, Radioimmunoassay, Antibodies, Pregnanediones, Progesterone

Related articles

Excretion of progesterone metabolites and estriol in faeces from pregnant women during ampicillin administration
Martin F et al., 1975 Journal of Steroid Biochemistry

Progesterone metabolites and estriol were determined in urine and faeces collected daily from three pregnant women (33–37 weeks) before and during ampicillin administration (2 g/day orally). Two of th...

Reproductive Endocrinology > Steroid Metabolism > Progesterone MetabolitesPregnancy > Pharmacology > Antibiotic Effects on HormonesDiagnostics > Hormone Measurement > Urinary and Fecal Steroids

Recollections of my life with progesterone
Allen WM, 1974 Gynecologic Investigation

This paper gives in narrative style those phases of the corpus luteum story in which the author was an active participant. The first part deals with the exciting days at the University of Rochester wh...

Reproductive Endocrinology > Progesterone > History of DiscoveryEthics/Philosophy > History of Reproductive Medicine > Hormone Research