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Glucocorticoid treatment decreases sera embryotoxicity in endometriosis patients

Gómez E et al., 1992

Fertility and sterility, 58(2), 284-289

DOI 10.1016/s0015-0282(16)55198-x PMID 1633892 Source

Abstract

Objective

To investigate the effect of short-term glucocorticoid administration on embryotoxicity of sera from infertile patients with mild to moderate endometriosis.

Design

Prospective longitudinal study. SETTING,

Patients

Eight infertile patients with mild to moderate endometriosis and a control group of eight infertile patients with tubal infertility were selected on the basis of laparoscopic examination.

Interventions

Basal (B) serum collection and day 1 (T1), day 3 (T2), day 6 (T3), and day 12 (T4) serum drawn after a 3-day glucocorticoid treatment in endometriosis patients.

Main outcome measure

Embryotoxicity of endometriosis sera, before and after glucocorticoid treatment, was investigated using a bioassay performed on two-cell mouse embryos. Interleukin 1 alpha (IL-1 alpha) and antismooth muscle, antimitochondrial, and antinuclear autoantibodies were also tested in these sera.

Results

At 50% concentration, endometriosis serum is embryotoxic in comparison with control; 0% versus 61% of the embryos reached the blastocyst stage at 72 hours, respectively (basal versus control, P less than 0.001). However, this embryotoxicity significantly decreases 12 days after glucocorticoid treatment in comparison with untreated sera; 32.4% versus 0% of the embryos reached blastocyst stage at 72 hours, respectively (T4 versus basal, P less than 0.001), although they did not reach nontoxic levels (greater than 50%). Interleukin 1 alpha was undetectable in all samples analyzed. In endometriosis sera, antismooth muscle antibody was detected.

Conclusions

At 50% concentration, serum from infertile patients with minimal to moderate endometriosis appears to be embryotoxic to the in vitro development of two-cell mouse embryos. However, this embryotoxicity significantly decreases 12 days after a 3-day treatment with glucocorticoids.

Topics

Endometriosis > Medical Management > HormonalEndometriosis > Pathophysiology > MechanismsInfertility > Female Factor > Endometriosis-RelatedInfertility > Unexplained > Treatment
endometriosis sera embryotoxicity treatment, glucocorticoid treatment endometriosis fertility, embryotoxic serum endometriosis patients, methylprednisolone endometriosis embryo development, endometriosis serum embryo toxicity mouse model, steroid treatment endometriosis infertility, autoantibodies endometriosis embryo implantation, glucocorticoid therapy endometriosis-related infertility, endometriosis immune factors embryo quality, corticosteroid endometriosis pregnancy outcomes

Cite this article

Gómez, E., Mir, A., De los Santos, M. J., Pellicer, A., & Simón, C. (1992). Glucocorticoid treatment decreases sera embryotoxicity in endometriosis patients. *Fertility and sterility*, *58*(2), 284-289. https://doi.org/10.1016/s0015-0282(16)55198-x

Keywords

Adult, Animals, Blastocyst, Blood, Culture Techniques, Endometriosis, Female, Fetal Death, Humans, Infertility, Longitudinal Studies, Methylprednisolone Hemisuccinate, Mice, Morula, Pregnancy, Prospective Studies

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