Abstract
Objective To examine the role of steroid hormone receptor compartmentalization in infertile women with "in-phase" or "out-of-phase" endometrium.
Design Nonrandomized prospective clinical study.
Setting A university clinic.
Patients Twenty-nine infertile patients without evidence of endometriosis, tubal factor, male factor, galactorrhea, or hyperandrogenism were enrolled. Sixteen patients had in-phase endometrium and a P level > or = 10 ng/mL (conversion factor to SI unit, 3.18) (group A). Four patients had out-of-phase endometrium and a P level > 10 ng/mL (group B). Four patients had in-phase endometrium and a P level < 10 ng/mL (group C), and five patients had out-of-phase endometrium and a P level < 10 ng/mL (group D).
Main Outcome Measures Each patient underwent determination of serum P and endometrial sampling on postovulatory days 6 to 9 based on serum LH measurement. Dating according to endometrial histology and biochemical assessment of estrogen receptor (ER) and P receptor (PR) were performed on each sample.
Results The level of cytosol ER was significantly lower in out-of-phase endometrium regardless of serum P level. There were no significant differences in the levels of cytosol PR and nuclear ER and PR among groups. In a long-term follow-up study, 6 of 29 patients became pregnant. The cytosol ER:PR ratio in these patients was found to fit a single straight regression line (y = 0.34x - 2.2).
Conclusion Out-of-phase endometrium probably depends on inadequate cytosol ER. An appropriate cytosol ER:PR ratio may be important to become pregnant.
estrogen progesterone receptor out-of-phase endometrium infertility, luteal phase defect endometrial receptor compartmentalization, cytosol estrogen receptor inadequate endometrial maturation, out-of-phase endometrium steroid hormone receptors female infertility, endometrial dating progesterone level receptor expression, cytosol ER PR ratio implantation pregnancy prediction, endometrial biopsy receptor analysis infertile women, luteal phase endometrium estrogen receptor deficiency, steroid receptor expression endometrial receptivity infertility, progesterone resistance endometrial dating discrepancy
PMID 7720943 7720943 DOI 10.1016/s0015-0282(16)57534-7 10.1016/s0015-0282(16)57534-7
Keywords
Adult, Cell Nucleus/metabolism, Cytosol/metabolism, Endometrium/metabolism, Estradiol/blood, Female, Follicle Stimulating Hormone/blood, Humans, Infertility, Female/metabolism, Luteal Phase/metabolism, Luteinizing Hormone/blood, Pregnancy, Progesterone/blood, Prospective Studies, Receptors, Estrogen/metabolism, Receptors, Progesterone/metabolism, Receptors, Estrogen, Receptors, Progesterone, Progesterone, Estradiol, Luteinizing Hormone, Follicle Stimulating Hormone