Abstract
Objective To evaluate the effect of vaginal lubricants Pre-Seed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity.
Design Prospective, comparative, in vitro study.
Setting Andrology laboratory at tertiary care hospital.
PATIENT(S): Thirteen normozoospermic donors.
INTERVENTION(S): Semen samples from 13 subjects were incubated in human tubal fluid media (HTF) controls and 10% (vol/vol) of Pre-Seed, FemGlide, Astroglide, and Replens lubricants. After 30 minutes, progressive sperm motility was assessed by light microscopy. Semen samples of 12 patients were placed in positive control (HTF), negative control (10% K-Y Jelly lubricant), and 10% vol/vol Pre-Seed and FemGlide lubricants. After 4 hours culture, spermatozoa were analyzed for percent DNA fragmentation index with use of the acridine orange-based sperm chromatin structure assay.
MAIN OUTCOME MEASURE(S): Sperm motility and percent DNA fragmentation index.
Results Percent motility did not differ significantly between HTF controls and Pre-Seed, whereas FemGlide, Replens, and Astroglide lubricants demonstrated a significant decrease in motility. There was no significant difference in percent DNA fragmentation index between the HTF controls and Pre-Seed, but a significant decline in sperm chromatin quality occurred with FemGlide and K-Y Jelly.
Conclusion Pre-Seed does not cause a significant decrease in progressive sperm motility or chromatin integrity in contrast to other lubricants used by couples.
vaginal lubricant effect sperm motility chromatin integrity, Pre-Seed lubricant sperm friendly fertility comparison study, Astroglide Replens FemGlide sperm motility decrease in vitro, vaginal lubricant sperm DNA fragmentation index prospective study, sperm chromatin structure assay SCSA lubricant effects, K-Y Jelly sperm chromatin quality damage, fertility-friendly lubricant comparison sperm function Agarwal, couples trying to conceive vaginal lubricant sperm toxicity, normozoospermic donors lubricant incubation progressive motility, Pre-Seed versus commercial lubricants sperm survival
PMID 17509584 17509584 DOI 10.1016/j.fertnstert.2007.02.050 10.1016/j.fertnstert.2007.02.050
Keywords
Cells, Cultured, Cellulose/analogs & Derivatives/pharmacology, Chromatin/drug Effects/metabolism, DNA Fragmentation/drug Effects, Female, Glycerol/pharmacology, Humans, Lipids/pharmacology, Lubricants/pharmacology, Male, Phosphates/pharmacology, Poloxamer/pharmacology, Polyethylene Glycols/pharmacology, Propylene Glycols/pharmacology, Sperm Motility/drug Effects, Spermatozoa/drug Effects/metabolism, Vaginal Creams, Foams, and Jellies/pharmacology, Astroglide, Chromatin, K-Y Jelly, Lipids, Lubricants, Phosphates, Propylene Glycols, Replens, Vaginal Creams, Foams, and Jellies, Femglide, Pre-seed, Poloxamer, Polyethylene Glycols, Cellulose, Glycerol