Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study
Fertility and sterility, 89(2), 375-379, 2008
Abstract
Objective
To evaluate the effect of vaginal lubricants Pre-Seed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity.
Design
Prospective, comparative, in vitro study.
Setting
Andrology laboratory at tertiary care hospital. PATIENT(S): Thirteen normozoospermic donors. INTERVENTION(S): Semen samples from 13 subjects were incubated in human tubal fluid media (HTF) controls and 10% (vol/vol) of Pre-Seed, FemGlide, Astroglide, and Replens lubricants. After 30 minutes, progressive sperm motility was assessed by light microscopy. Semen samples of 12 patients were placed in positive control (HTF), negative control (10% K-Y Jelly lubricant), and 10% vol/vol Pre-Seed and FemGlide lubricants. After 4 hours culture, spermatozoa were analyzed for percent DNA fragmentation index with use of the acridine orange-based sperm chromatin structure assay. MAIN OUTCOME MEASURE(S): Sperm motility and percent DNA fragmentation index.
Results
Percent motility did not differ significantly between HTF controls and Pre-Seed, whereas FemGlide, Replens, and Astroglide lubricants demonstrated a significant decrease in motility. There was no significant difference in percent DNA fragmentation index between the HTF controls and Pre-Seed, but a significant decline in sperm chromatin quality occurred with FemGlide and K-Y Jelly.
Conclusion
Pre-Seed does not cause a significant decrease in progressive sperm motility or chromatin integrity in contrast to other lubricants used by couples.
Topics
Cite this article
Agarwal, A., Deepinder, F., Cocuzza, M., Short, R. A., & Evenson, D. P. (2007). Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study. *Fertility and sterility*, *89*(2), 375-379. https://doi.org/10.1016/j.fertnstert.2007.02.050
Agarwal A, Deepinder F, Cocuzza M, Short RA, Evenson DP. Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study. Fertil Steril. 2007;89(2):375-379. doi:10.1016/j.fertnstert.2007.02.050
Agarwal, A., et al. "Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study." *Fertility and sterility*, vol. 89, no. 2, 2007, pp. 375-379.
Keywords
Cells, Cultured, Cellulose, Chromatin, DNA Fragmentation, Female, Glycerol, Humans, Lipids, Lubricants, Male, Phosphates, Poloxamer, Polyethylene Glycols, Propylene Glycols, Sperm Motility, Spermatozoa, Vaginal Creams, Foams, and Jellies, Astroglide, K-Y Jelly, Replens, Femglide, Pre-seed